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The technology behind

The secret to finding high quality nanobodies lies in the in the strength of the libraries. Immunordic has developed technology that allows creation of vastly diverse nanobody libraries quick and inexpensively. We call it Evolink technology.

On a robust foundation of libraries developed through Evolink technology we have build an advanced process of screening and validation.  An optimized phage display pipeline allow for rapid screening and high throughput ELISA serve for solid validation.


 

EvoLink

Evolink 

Anchored in Immunordic’s core is the proprietary Evolink technology.

Evolink, is a pioneering approach to generating extensive and complex nanobody libraries with minimal resources. This approach facilitates the rapid and cost-effective identification of nanobodies.

 

Evolink employs four parental nanobody sequences, which are humanized to eliminate immunogenicity in downstream applications. These sequences serve as the foundation for all nanobody variants, and extensive mutagenesis is applied to the three hypervariable regions (CDRs) using site-directed mutagenesis.

 

Evolink is harnessing the power of site-directed mutagenesis while upholding precision and eliminating randomness, thereby ensuring high quality of each nanobody.

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In house library

Readily available for screening and selection

Phage display

Screening and selection of nanobodies

ELISA

Validation of high-affinity nanobodies

for all targets

Sequencing

Verification that the top performing nanobodies are different and intact

Production

Large-scale production of nanobodies of interest

In house library

In house library

We have developed a highly efficient and powerful in-house nanobody phage display library consisting of a vast collection (10^11) of nanobodies, and each nanobody is engineered for stability and high-affinity binding using our Evolink technology. The readily available nanobody library requires no processes and the screening thus commences immediately upon receiving the antigen. 

Phage display

Upon receiving the target protein of your choice, we commence the screening process, which comprises of two to five rounds of phage display biopanning. The use of our optimized phage display pipeline facilitates the most rapid and reliable selection of functional nanobodies from our extensive library.  Following the initial selection, we conduct small-scale production, which will subsequently undergo ELISA validation.
 

Phage display
Elisa

Elisa

Upon completion of the phage display biopanning rounds and small-scale production, we identify nanobody candidates with the highest binding affinities via ELISA. This methodological approach ensures the identification of nanobodies with exceptional binding characteristics and absence of production liabilities, thereby enabling the production of high-quality products tailored to your specific application.

After the completion of the ELISA validation process, the most promising nanobodies can be produced and delivered to you for further assessment in your applications. In the event that a significant quantity of the product is required, a larger scale production is available through special ordering. For additional information on this matter, please contact Immunordic.

Sequencing

Sequencing

Once the validation is complete, we proceed to sequence the best nanobody candidates, taking great care to ensure their diversity and integrity. By employing these rigorous procedures, we provide you with nanobodies that meet the most stringent standards, ensuring optimal performance and reliability for your applications.

Production

Production

Once the ideal nanobody candidates have been identified and validated, we possess the capability to produce them using our specialized production strain. Leveraging our advanced production techniques, we cultivate the strain under controlled conditions to achieve a substantial yield of the desired nanobodies. Our optimized production process ensures that the nanobodies are generated efficiently and in significant quantities, enabling you to access a robust supply for your specific applications.

Option coming soon

Customization

At Immunordic we develop our products constantly in order to deliver the best possible solutions for our customers. Therefore, we will soon be able to offer customisation of our nanobodies to better accommodate various requests on our products. Keep informed on our website. 

Option coming soon

Affinity maturation

Once a nanobody that demonstrates efficacy in application has been identified, you will soon have have the opportunity to avail our unique affinity maturation technology. This process involves creating a new library based on the hit nanobody using our Evolink technology, which will then be utilized in another round of screening and validation.

We are here to help.

At Immunordics, we have solutions that suits most needs, but we are aware that you might need specific information regarding your business in terms of choosing the right nanobodies. If you have any questions about which solution best matches you or your business’ needs, we are always ready to help.

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